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KMID : 0043319990220020130
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Archives of Pharmacal Research
1999 Volume.22 No. 2 p.130 ~ p.136
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Interferon-r and Lipopolysaccaride Induce Mouse Guanylate-Binding Protein 3 (mGBP3) Expression in the Murine Macrophage Cell Line RAW264-7
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Han Byung-Hee
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Abstract
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Mouse guanylate-binding protein 3 (mGBP3) is a 71-kDa GTPase which belongs to GTP-binding protein family. The present study showed that the expression of mGBP3 transcript was readily induced in a dose dependent fashion in the macrophage cell line RAW264.7 treated with either or lipopolysaccaride (LPS). The expression of mGBP3 protein was also apparent by 4 and 6 h after the treatment of cells with IFN-\gamma (100 U/ml) or LPS () , and remained at palteau for at least 24 h. Cycloheximide () had no effect on the or LPS-induced mGBP3 expression, suggesting that the mGBP3 induction did not require further protein synthesis. Interestingly, a protein kinase C (PKC) inhibitor staurosporine (50 nM) abolished the induction of mGBP3 expression by LPS, but not by . These findings suggest that mGBP3 may be involved in the macrophage activation process and both IFN-\gamma and LS induce the mGBP3 expression through distinct signal transduction pathways.
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KEYWORD
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Guanylate-binding protein 3, Gene expression, Lipopolysaccaride, Macrophage activation, Staurosporine, AG490
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